Selected clones provided useful markers to follow B. cereus cells expressing GFP from a 10 000-fold excess of non-expressing cells. Flow cytometry and cell sorting efficiently separated B. We identified 21 clones as fluorescing in the initial screen, and further characterized these clones by restriction analysis, sequencing, and quantification of fluorescence intensity. GFP expression was detected by visual inspection with a fluorimager. cereus UW85 using a 96-well microtiter dish assay. A portion of the library (988 clones) was screened for GFP expression in B. We constructed a library in pAD123, which consists of 29 000 clones containing chromosomal DNA from B. The plasmid replicates and confers drug resistance in both Escherichia coli and B. This plasmid contains a promoterless gene that encodes a mutant version of the green fluorescent protein, GFPmut3a, that is optimized for fluorescence-activated cell sorting. The Edit menu is used to load a different sequence, start a new sequence, copy the sequence and to discard all changes.We constructed a promoter-trap plasmid, pAD123, for Bacillus cereus. The Undo and Redo menu selections can be used to undo and redo changes to the sequence. The Show All Dances checkbox will display all available dances. The default list of available dances is set in edit dances. The dances may also be moved using the up and down keys. The Move Up and Move Down buttons will repeat if held down. Use the Move Up, Remove and Move Down buttons to adjust the sequence. Select a dance or dances on the right and select the <- Select button to add them to the sequence. A sequence can also be used to create the sequence of dances for an entire evening. The sequence editor allows a sequence of any length. The sequence of dances will be: Waltz, Tango, Viennese Waltz, Foxtrot, Quickstep and repeat until stopped.
BallroomDJ playlists can be created to use a sequence to select the dances in the sequence order and the BallroomDJ program will choose the songs based on those dances. A sequence is a list of dances that will repeat over and over again. (Updated 2016-8 BallroomDJ version 3.1.9)Įdit Sequences allows creation of a sequence.
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